LOGO CENTRE LNC

Lipides, Nutrition, Cancer
SÉminaires LNC
AnnÉe 2018

Intervenant : Dr Yegor Vassetzky

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Date :25 Mai 2018 14h30
Lieu : Faculté de Médecine - Amphi Courtois
Sujet :

How viruses shape B-cells for oncogenesis

Background: Tat protein of human immunodeficiency virus (HIV-1) is released by infected T-cells and is able to penetrate other uninfected cells, leading to the development of different pathologies, including cancer. HIV-infected patients, even those receiving combined antiretroviral therapy (cART) develop Hodgkin lymphoma and Burkitt lymphoma at high frequency. To elucidate the mechanisms of HIV Tat induced oncogenesis, we have treated primary human B cells with low doses of purified Tat protein. Tat induced oxidative DNA damage1, overexpression of oncogenes and profoundly rearranged nuclear architecture of B-cells, changing nuclear positions of gene loci and altering epigenetic marks2. We now develop a CRISPR/Cas9-based model for Tat-mediated oncogenesis that will contribute to the understanding of the pathogenesis of B-cell lymphomas at molecular level and may be instrumental in exploring novel therapeutic methods for lymphoma therapy and prevention.

 

Selected publications:

1. El-Amine, R. et al. HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production. , Redox Biol. (2018)

2. Germini, D. et al. HIV Tat induces a prolonged MYC relocalization next to IGH in circulating B-cells., Leukemia (2017)

Invitation: Oleg Demidov - UMR1231

Le séminaire aura lieu à la faculté de médecine -Amphi Courtois - à 14h30

Renseignements - omicheau@u-bourgogne.fr Tel: 03 80 39 34 68